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T4 DNA Ligase invitrogen试剂

T4 DNA Ligase invitrogen试剂
T4 DNA Ligase invitrogen试剂

T4 DNA Ligase invitrogen试剂
货号:15224025【15224-025】
单位规格:100units

产品型号:15224025

联系方式

86-021-65672052

产品先容

Invitrogen试剂15224025现货供应

T4 DNA Ligase invitrogen试剂
货号:15224025【15224-025】
单位规格:100units

产品报价:1363元 【折扣价请咨询!】

描述

T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.

Applications
Cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA

Source
Purified from E. coli lambda lysogen NM989

Performance and quality testing
Endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested

Unit definition
One unit catalyzes the exchange of 1 nmol 32P-labeled pyrophosphate into ATP in 20 min at 37°C. One unit is equal to approximay 300 cohesive-end ligation units.

Unit reaction conditions
66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 10 mM DTT, 66 µM ATP, 3.3 µM 32P-labeled pyrophosphate, and enzyme in 0.1 mL for 20 min at 37°C.

规格

Enzyme:Ligase
Product Size:100 units
Concentration:1 U⁄µl
Shipping Condition:Dry Ice

内容及储存

T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

Invitrogen试剂15224025现货供应

T4 DNA Ligase invitrogen试剂
货号:15224025【15224-025】
单位规格:100units

产品报价:1363元 【折扣价请咨询!】

invitrogen50rxn  18080-051    SuperScript III First-Strand 
invitrogen40000U 28025-013 M-MLV  
invitrogen25ug18418-012  Oligo(dT)12-18 
invitrogen 25ug18418-020   Oligo(dT)20 
invitrogen5000u 10777-019   RNaseOUT
invitrogen 100U 15224-017  T4 DNA Ligase(1U/ul)
invitrogen  500U 15224-025 T4 DNA Ligase(1U/ul)
invitrogen 1ml 18427-088   10mM dNTP Mix

产品相关关键字: Invitrogen试剂现货 gibco试剂现货 15224017现货 15224025供应 生物试剂

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