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新葡新京娱乐场>澳门新浦京8455com>>Promega试剂>M4103 T4 Polynucleotide Kinase Promega试剂

M4103 T4 Polynucleotide Kinase Promega试剂

M4103 T4 Polynucleotide Kinase Promega试剂
M4103 T4 Polynucleotide Kinase Promega试剂

M4103 T4 Polynucleotide Kinase Promega试剂
规格:1000u
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产品先容

M4103 T4 Polynucleotide Kinase Promega试剂 原装正品,质量保证,现货供应

规格:1000u

M4103 T4 Polynucleotide Kinase Promega试剂  产品描述:

其它组分:

 

Kinase 10X Buffer【子货号:C131A,包装:2 x 500μl,,运保温度:-20℃】

T4 Polynucleotide Kinase【子货号:M410B,包装:1 x 1,000u,,运保温度:-20℃】

 

 

描述:

 

说明

 

T4 Polynucleotide Kinase(T4 多核苷酸激酶) 催化ATP 的γ -磷酸转移到多聚核苷酸的5`- 末端或带有5`-OH 的单核苷酸的反应。该酶从重组E. coli 纯化而来, 可用于后续操作,如连接前的RNA、DNA 以及合成的寡聚核苷酸的磷酸化。

 

特点

 

• 高温失活:T4 Polynucleotide Kinase 可在68℃加热10 分钟失活。

• 提供10× 反应缓冲液:700mM Tris-HCl (pH 7.6,25℃ ),100mM MgCl2,50mM DTT。

• 蓝/白克隆验证:Promega 的蓝/ 白克隆检测为用于克隆的酶提供了高水平的质量控制。

• 可定制或批量订购:从以下获得更多关于该产品的自定义选项:www.promega。。com/myway/。

 

 

 

原厂资料:

 

T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. The enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.

Features - Benefits

  • May Be Heat-Inactivated: T4 Polynucleotide Kinase may be inactivated by heating at 68°C for 10 minutes.
  • Provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT.
  • Blue/White Cloning Qualified: Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.
  • Choose Your Configuration: Learn more about our custom options for this product at: www.promega。。com/custom/

Applications

  • 5´ end-labeling of single- or double-stranded DNA and RNA molecules for use as probes, for sequencing or for DNA-protein footprinting.
  • Phosphorylation of DNA prior to cloning.

M4103 T4 Polynucleotide Kinase Promega试剂  相关产品列表:

Promega 系列
序号货号  名称 规格
1M1801  T4连接酶         100U 
2M1804      T4连接酶      500U
3M1701      M-MLV              10000U 
4M1705      M-MLV             50000U 
5M5101      AMV                    300U 
6M5108      AMV             1000U  
7M7741      Pfu                      100U
8M7745      Pfu            500U  
9C1101      Olig(dt)15 Primer          20ug 
10M3001      Go-Taq                   100U  
11M3005      Go-Taq                 500U    
12M3008      Go-Taq                2500U     
13U1511      dNTP                   200μl   
14U1515      dNTP                   1000μl  
15A1360      pGEM-T easy              1.2ug   
16A3600      pGEM-T                     1.2ug 
17C1181      Random Primers              20μg  
18N2511      Recombinant RNasin           2500U 
19N2515      Recombinant RNasin      10000U  
20N2611      RNasin Plus                2500U  
21N2615      RNasin Plus              10000U   
22N2111      RNasin                   2500U     
23N2115      RNasin                  10000U   
24M1871      TdT                       300U 
25M1875      TdT                      1500U 
26M2825      CIAP                       1000U  
27M1821      CIAP           1000U            
28M2201      Klenow I      150U        
29M2206      Klenow I        500U           
30M4211      T4 DNA Polymerase  100U      
31M4215      T4 DNA Polymerase   500U          
32M4101      T4 Polynucleotide Kinase 100U          
33M4103      T4 Polynucleotide Kinase  1000U         

产品相关关键字: Promega试剂

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 相关同类产品:
Promega普洛麦格C1101 Oligo(dT)15 Primer 20ug 现货  GoTaq DNA Polymerase 100u 5u/μl Promega普洛麦格M3001现货  GoTaq DNA Polymerase 500u 5u/μl Promega普洛麦格M3005现货 
M4103 T4 Polynucleotide Kinase Promega试剂  M4101 T4 Polynucleotide Kinase Promega试剂  M4215 T4 DNA Polymerase Promega试剂 
M4211 T4 DNA Polymerase Promega试剂  M2206 Klenow I Promega试剂  M2201 Klenow I Promega试剂 

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