M4103 T4 Polynucleotide Kinase Promega试剂
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M4103 T4 Polynucleotide Kinase Promega试剂规格:1000u上海桥星现货供应进口透析袋,密理博ECL发光液,B27无血清培养基,N2培养基,紫外酶标板,Gibco胶原酶,Trizol一步法试剂,反转录酶试剂盒,脂质体2000转染试剂,Roche原装潮霉素B,欢迎各单位及采购。
产品型号:
联系方式
86-021-65672052
M4103 T4 Polynucleotide Kinase Promega试剂 原装正品,质量保证,现货供应
规格:1000u
M4103 T4 Polynucleotide Kinase Promega试剂 产品描述:
其它组分:
Kinase 10X Buffer【子货号:C131A,包装:2 x 500μl,,运保温度:-20℃】
T4 Polynucleotide Kinase【子货号:M410B,包装:1 x 1,000u,,运保温度:-20℃】
描述:
说明 |
| T4 Polynucleotide Kinase(T4 多核苷酸激酶) 催化ATP 的γ -磷酸转移到多聚核苷酸的5`- 末端或带有5`-OH 的单核苷酸的反应。该酶从重组E. coli 纯化而来, 可用于后续操作,如连接前的RNA、DNA 以及合成的寡聚核苷酸的磷酸化。
|
特点 |
| • 高温失活:T4 Polynucleotide Kinase 可在68℃加热10 分钟失活。 |
原厂资料:
T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. The enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.
Features - Benefits
- May Be Heat-Inactivated: T4 Polynucleotide Kinase may be inactivated by heating at 68°C for 10 minutes.
- Provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT.
- Blue/White Cloning Qualified: Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.
- Choose Your Configuration: Learn more about our custom options for this product at: www.promega。。com/custom/
Applications
- 5´ end-labeling of single- or double-stranded DNA and RNA molecules for use as probes, for sequencing or for DNA-protein footprinting.
- Phosphorylation of DNA prior to cloning.
M4103 T4 Polynucleotide Kinase Promega试剂 相关产品列表:
Promega 系列 | ||
序号 | 货号 名称 | 规格 |
1 | M1801 T4连接酶 | 100U |
2 | M1804 T4连接酶 | 500U |
3 | M1701 M-MLV | 10000U |
4 | M1705 M-MLV | 50000U |
5 | M5101 AMV | 300U |
6 | M5108 AMV | 1000U |
7 | M7741 Pfu | 100U |
8 | M7745 Pfu | 500U |
9 | C1101 Olig(dt)15 Primer | 20ug |
10 | M3001 Go-Taq | 100U |
11 | M3005 Go-Taq | 500U |
12 | M3008 Go-Taq | 2500U |
13 | U1511 dNTP | 200μl |
14 | U1515 dNTP | 1000μl |
15 | A1360 pGEM-T easy | 1.2ug |
16 | A3600 pGEM-T | 1.2ug |
17 | C1181 Random Primers | 20μg |
18 | N2511 Recombinant RNasin | 2500U |
19 | N2515 Recombinant RNasin | 10000U |
20 | N2611 RNasin Plus | 2500U |
21 | N2615 RNasin Plus | 10000U |
22 | N2111 RNasin | 2500U |
23 | N2115 RNasin | 10000U |
24 | M1871 TdT | 300U |
25 | M1875 TdT | 1500U |
26 | M2825 CIAP | 1000U |
27 | M1821 CIAP | 1000U |
28 | M2201 Klenow I | 150U |
29 | M2206 Klenow I | 500U |
30 | M4211 T4 DNA Polymerase | 100U |
31 | M4215 T4 DNA Polymerase | 500U |
32 | M4101 T4 Polynucleotide Kinase | 100U |
33 | M4103 T4 Polynucleotide Kinase | 1000U |
产品相关关键字: Promega试剂
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