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M2201 Klenow I Promega试剂

M2201 Klenow I  Promega试剂
M2201 Klenow I  Promega试剂

M2201 Klenow I Promega试剂
规格:150u
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产品型号:

联系方式

86-021-65672052

产品先容

M2201 Klenow I  Promega试剂? 原装正品,质量保证,现货供应

规格:150u

M2201 Klenow I  Promega试剂?  产品描述:

其它组分:

 

DNA Polymerase 10X Buffer【子货号:M195A,包装:1 x 1ml,,运保温度:-20℃】

DNA Polymerase I Large (Klenow) Fragment【子货号:M220A,包装:1 x 150u,,运保温度:-20℃】

 

 

描述:

 

说明

 

DNA Polymerase I Large (Klenow) Fragment (DNA 聚合酶I 大(Klenow) 片段) 是DNA 依赖的DNA 聚合酶,它没有完整的E. coli DNA Polymerase I 所具有的 5` → 3` 外切酶活性,但保留了5` → 3` 聚合酶、3` → 5` 外切酶及链置换活性。该酶是DNA 聚合酶I 的C- 末端片段,68kDa。Klenow 片段5` → 3` 聚合酶活性经常被用于以非标记的或标记的dNTPs 补平5`- 突出端、单链或双链DNA 模板测序、合成寡核苷酸进行体外突变、cDNA第二链合成以及制备单链DNA探针。3` → 5` 外切酶活性可用于从3`- 突出端产生平末端。

 

特点

 

• 灵活性:DNA Polymerase I Large (Klenow) Fragment 可用于多种分子实验。在多种Promega 1× 内切酶缓冲液中它都能保持活性。

• 高温灭活:DNA Polymerase I Large (Klenow) Fragment 在75℃加热10 分钟失活。

• 提供10× 反应缓冲液:500mM Tris-HCl (pH 7.2,25℃ ),100mM MgSO4,1mM DTT。

• 可定制或批量订购:从以下获得更多关于该产品的定制选项:www.promega。。com/myway/。

 

 

 

原厂资料:

 

DNA Polymerase I Large (Klenow) Fragment is a DNA-dependent DNA polymerase that lacks the 5´→3´ exonuclease activity of intact E. coli DNA Polymerase I but retains its 5´→3´ polymerase, 3´→5´ exonuclease and strand displacement activities. The enzyme is a 68kDa C-terminal fragment of DNA Polymerase I. The 5´→3´ polymerase activity of Klenow Fragment can be used to fill in 5´-protruding ends with unlabeled or labeled dNTPs, to sequence single- or double-stranded DNA templates, for in vitro mutagenesis using synthetic oligonucleotides, for cDNA second-strand synthesis and to generate single-stranded DNA probes. The 3´→5´ exonuclease activity can be used to generate blunt ends from a 3´-overhang.

Features - Benefits

Flexible: DNA Polymerase I Large (Klenow) Fragment may be used in a variety of molecular applications. It is also active in many Promega 1X restriction enzyme buffers.
May Be Heat-Inactivated: DNA Polymerase I Large (Klenow) Fragment is inactivated by heating at 75°C for 10 minutes.
Provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega。。com/custom/

Applications

Filling-in or labeling 3´ ends of 5´ overhanging double-stranded DNA.
Second-strand cDNA synthesis.
DNA sequencing by the dideoxy method.

References

Anderson, S. et al. (1980) Nucl. Acids Res. 8, 1731–43.
Sanger, F. et al. (1977) Proc. Natl. Acad. Sci. USA 74, 5463–7.
Wallace, R.B. et al. (1980) Science 209, 1396–400.
Houdebine, L.M. (1976) Nucl. Acids Res. 3, 615–30.
Feinberg, A.P. and Vogelstein, B. (1983) Anal. Biochem. 132, 6–13.
Tabor, S. and Struhl, K. (1987) In: Current Protocols in Molecular Biology, Ausubel, F.M. et al., eds., John Wiley and Sons, New York, NY.
Joyce, C.M. and Grindley, N.D.F. (1983) Proc. Natl. Acad. Sci. USA 80, 1830–4.

M2201 Klenow I  Promega试剂?  相关产品列表:

Promega 系列
序号货号  名称 规格
1M1801  T4连接酶         100U 
2M1804      T4连接酶      500U
3M1701      M-MLV              10000U 
4M1705      M-MLV             50000U 
5M5101      AMV                    300U 
6M5108      AMV             1000U  
7M7741      Pfu                      100U
8M7745      Pfu            500U  
9C1101      Olig(dt)15 Primer          20ug 
10M3001      Go-Taq                   100U  
11M3005      Go-Taq                 500U    
12M3008      Go-Taq                2500U     
13U1511      dNTP                   200μl   
14U1515      dNTP                   1000μl  
15A1360      pGEM-T easy              1.2ug   
16A3600      pGEM-T                     1.2ug 
17C1181      Random Primers              20μg  
18N2511      Recombinant RNasin           2500U 
19N2515      Recombinant RNasin      10000U  
20N2611      RNasin Plus                2500U  
21N2615      RNasin Plus              10000U   
22N2111      RNasin                   2500U     
23N2115      RNasin                  10000U   
24M1871      TdT                       300U 
25M1875      TdT                      1500U 
26M2825      CIAP                       1000U  
27M1821      CIAP           1000U            
28M2201      Klenow I      150U        
29M2206      Klenow I        500U           
30M4211      T4 DNA Polymerase  100U      
31M4215      T4 DNA Polymerase   500U          
32M4101      T4 Polynucleotide Kinase 100U          
33M4103      T4 Polynucleotide Kinase  1000U         

产品相关关键字: Promega试剂

返回

 相关同类产品:
Promega普洛麦格C1101 Oligo(dT)15 Primer 20ug 现货  GoTaq DNA Polymerase 100u 5u/μl Promega普洛麦格M3001现货  GoTaq DNA Polymerase 500u 5u/μl Promega普洛麦格M3005现货 
M4103 T4 Polynucleotide Kinase Promega试剂  M4101 T4 Polynucleotide Kinase Promega试剂  M4215 T4 DNA Polymerase Promega试剂 
M4211 T4 DNA Polymerase Promega试剂  M2206 Klenow I Promega试剂  M2201 Klenow I Promega试剂 

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