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M1821 CIAP Promega试剂

M1821 CIAP Promega试剂
M1821 CIAP Promega试剂

M1821 CIAP Promega试剂
规格:1000u
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产品型号:

联系方式

86-021-65672052

产品先容

M1821 CIAP Promega试剂 原装正品,质量保证,现货供应

规格:1000u

M1821 CIAP Promega试剂 产品描述:

其它组分:

 

Alkaline Phosphatase, Calf Intestinal【子货号:M182A,包装:1 x 1,000u,,运保温度:-20℃】

Alkaline Phosphatase 10X Buffer【子货号:M183A,包装:2 x 500μl,,运保温度:-20℃】

 

 

描述:

 

说明

 

Alkaline Phosphatase, Calf Intestinal (CIAP,小牛肠碱性磷酸酶) 催化从DNA、RNA、核糖及脱氧核糖核苷三磷酸上水解5`- 磷酸基团的反应。该酶通过消除5`- 末端磷酸基团,阻止线性化的克隆载体DNA 重新环化及再连接,可以用于DNA 或RNA 的5` 磷酸化末端脱磷酸,以利于用[32P]ATP 及T4 Polynucleotide Kinase(T4 多聚核苷酸激酶) 进行标记。CIAP 对于5` 突出端、5` 凹端及平末端都具有活性。

 

特点

 

• 提供高浓度产品:Cat.# M2825 包含1,000 单位20u/μl 的CIAP。

• 蓝/ 白克隆验证:Promega 的蓝/ 白克隆检测为用于克隆的酶提供了高水平的质量控制。

• 提供10× 反应缓冲液:0.5M Tris-HCl (pH 9.3,25℃ ),10mM MgCl2,1mM ZnCl2,10mM 精胺。

 

 

 

原厂资料:

 

Alkaline Phosphatase, Calf Intestinal (CIAP), catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vector DNA by removing phosphate groups from both 5´-termini and may also be used for the dephosphorylation of 5´ phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP and T4 Polynucleotide Kinase. CIAP is active on 5´ overhangs, 5´ recessed and blunt ends.

Features - Benefits

  • Available at High Concentration: Cat.# M2825 contains 1,000 units of CIAP at 20u/μl.
  • Blue/White Cloning Qualified: Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.
  • Provided with 10X Reaction Buffer: 0.5M Tris-HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM spermidine.

Applications

  • Preventing religation of linearized cloning vehicle DNA by removing phosphate groups from both 5´ termini.
  • Removing 5´ phosphate groups prior to end-labeling with T4 Polynucleotide Kinase.

For more information, see the Protocols & Applications Guide.

References

  1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  2. Seeburg, P.H. et al. (1977) Nature 270, 486–94.
  3. Ullrich, A. et al. (1977) Science 196, 1313–9.
  4. Meyerowitz, E.M. et al. (1980) Gene 11, 271–82.
  5. Grosveld, F.G. et al. (1981) Gene 13, 227–37.

M1821 CIAP Promega试剂 相关产品列表:

Promega 系列
序号货号  名称 规格
1M1801  T4连接酶         100U 
2M1804      T4连接酶      500U
3M1701      M-MLV              10000U 
4M1705      M-MLV             50000U 
5M5101      AMV                    300U 
6M5108      AMV             1000U  
7M7741      Pfu                      100U
8M7745      Pfu            500U  
9C1101      Olig(dt)15 Primer          20ug 
10M3001      Go-Taq                   100U  
11M3005      Go-Taq                 500U    
12M3008      Go-Taq                2500U     
13U1511      dNTP                   200μl   
14U1515      dNTP                   1000μl  
15A1360      pGEM-T easy              1.2ug   
16A3600      pGEM-T                     1.2ug 
17C1181      Random Primers              20μg  
18N2511      Recombinant RNasin           2500U 
19N2515      Recombinant RNasin      10000U  
20N2611      RNasin Plus                2500U  
21N2615      RNasin Plus              10000U   
22N2111      RNasin                   2500U     
23N2115      RNasin                  10000U   
24M1871      TdT                       300U 
25M1875      TdT                      1500U 
26M2825      CIAP                       1000U  
27M1821      CIAP           1000U            
28M2201      Klenow I      150U        
29M2206      Klenow I        500U           
30M4211      T4 DNA Polymerase  100U      
31M4215      T4 DNA Polymerase   500U          
32M4101      T4 Polynucleotide Kinase 100U          
33M4103      T4 Polynucleotide Kinase  1000U         

产品相关关键字: Promega试剂

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 相关同类产品:
Promega普洛麦格C1101 Oligo(dT)15 Primer 20ug 现货  GoTaq DNA Polymerase 100u 5u/μl Promega普洛麦格M3001现货  GoTaq DNA Polymerase 500u 5u/μl Promega普洛麦格M3005现货 
M4103 T4 Polynucleotide Kinase Promega试剂  M4101 T4 Polynucleotide Kinase Promega试剂  M4215 T4 DNA Polymerase Promega试剂 
M4211 T4 DNA Polymerase Promega试剂  M2206 Klenow I Promega试剂  M2201 Klenow I Promega试剂 

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