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M1821 CIAP Promega试剂 原装正品，质量保证，现货供应

规格：1000u

M1821 CIAP Promega试剂 产品描述：

其它组分：

Alkaline Phosphatase, Calf Intestinal【子货号：M182A，包装：1 x 1,000u，，运保温度：-20℃】

Alkaline Phosphatase 10X Buffer【子货号：M183A，包装：2 x 500μl，，运保温度：-20℃】

描述：

原厂资料：

Alkaline Phosphatase, Calf Intestinal (CIAP), catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vector DNA by removing phosphate groups from both 5´-termini and may also be used for the dephosphorylation of 5´ phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP and T4 Polynucleotide Kinase. CIAP is active on 5´ overhangs, 5´ recessed and blunt ends.

Features - Benefits

• Available at High Concentration: Cat.# M2825 contains 1,000 units of CIAP at 20u/μl.
• Blue/White Cloning Qualified: Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.
• Provided with 10X Reaction Buffer: 0.5M Tris-HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM spermidine.

Applications

• Preventing religation of linearized cloning vehicle DNA by removing phosphate groups from both 5´ termini.
• Removing 5´ phosphate groups prior to end-labeling with T4 Polynucleotide Kinase.

For more information, see the Protocols & Applications Guide.

References

1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
2. Seeburg, P.H. et al. (1977) Nature 270, 486–94.
3. Ullrich, A. et al. (1977) Science 196, 1313–9.
4. Meyerowitz, E.M. et al. (1980) Gene 11, 271–82.
5. Grosveld, F.G. et al. (1981) Gene 13, 227–37.

M1821 CIAP Promega试剂 相关产品列表：

产品相关关键字： Promega试剂