N2115 RNasin Promega试剂
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N2115 RNasin Promega试剂规格：10000u上海桥星现货供应进口透析袋,密理博ECL发光液,B27无血清培养基,N2培养基,紫外酶标板，Gibco胶原酶，Trizol一步法试剂,反转录酶试剂盒,脂质体2000转染试剂,Roche原装潮霉素B，欢迎各单位及采购。
产品型号：
联系方式
86-021-65672052
N2115 RNasin Promega试剂?
原装正品，质量保证，现货供应
规格：10000u
N2115 RNasin Promega试剂? 产品描述：
组分：
RNasin® Ribonuclease Inhibitor【子货号：N211B，包装：1 x 10,000u，，运保温度：–20°C】
描述：
说明 |
| 天然的和重组的RNasin® 核酸酶抑制剂具有广谱的抑制RNase 活性的性质，包括抑制中性型(neutral type) 真核RNase。该抑制剂的分子量为50kDa，按1:1 的比例与RNase 非共价结合发挥抑制作用。RNasin® Ribonuclease Inhibitor 与RNase( 如RNase A) 结合的Ki 值大约是10-14M。Promega 提供两种不同的抑制剂：天然的RNasin® Ribonuclease Inhibitor 和重组的RNasin® Ribonuclease Inhibitor。两种产品均通过离子交换和亲和层析相结合的纯化方法纯化得到。
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特点 |
| • 抑制常见的真核RNase：RNasin® Ribonuclease Inhibitor 具有广谱RNase 抑制特性，包括 RNase A、 RNase B、 RNase C 和人类胎盘RNase，不抑制RNase T1、 S1 核酸酶、曲霉(Aspergillus ) 中的 RNase 以及RNase H、RNase ONE® Ribonuclease。
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原厂资料：
Native RNasin® Inhibitor is a 50kDa protein that inhibits RNase A family and human placental RNases by noncovalently binding to RNases in a 1:1 ratio. Recombinant RNasin® Inhibitor does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, RNase H, RNase ONE™ Ribonuclease and enzymes for downstream applications such as GoScript™ Reverse Transcriptase, AMV/M-MLV reverse transcriptases, SP6, T7/T3 RNA polymerase and Taq DNA polymerases.
Features - Benefits
- Inhibits Common Eukaryotic RNases: Carries broad-spectrum RNase inhibitory properties.
- Compatible: Does not inhibit SP6, T7 or T3 RNA Polymerase; GoScript™, AMV or M-MLV Reverse Transcriptase; or Taq DNA polymerase.
- Broad pH Range (pH 5–8): Offers flexibility in downstream assays.
Applications
- RT-PCR.
- Useful in any applications where eukaryotic RNase contamination is a potential problem.
- Protection of mRNA in cDNA synthesis reactions.
- In vitro transcription/translation.
- Increase yield and activity of polysomes.
- Improvement of in vitro virus replication.
- Improvement of RNA translation in homologous systems.
- Performing RNase-free antibody staining.
- Enhancement of RNA yields from Riboprobe® System RNA synthesis reactions.
References
- Blackburn, P. and Moore, S. (1982) In: The Enzymes, Vol. XV, Part B.
- Blackburn, P. et al. (1977) J. Biol. Chem. 252, 5904–10.
- Lee, F.S. et al. (1989) Biochemistry 28, 219–24.
- Lee, F.S. et al. (1989) Biochemistry 28, 225–30.
N2115 RNasin Promega试剂? 相关产品列表：
| Promega 系列 | ||
| 序号 | 货号 名称 | 规格 |
| 1 | M1801 T4连接酶 | 100U |
| 2 | M1804 T4连接酶 | 500U |
| 3 | M1701 M-MLV | 10000U |
| 4 | M1705 M-MLV | 50000U |
| 5 | M5101 AMV | 300U |
| 6 | M5108 AMV | 1000U |
| 7 | M7741 Pfu | 100U |
| 8 | M7745 Pfu | 500U |
| 9 | C1101 Olig(dt)15 Primer | 20ug |
| 10 | M3001 Go-Taq | 100U |
| 11 | M3005 Go-Taq | 500U |
| 12 | M3008 Go-Taq | 2500U |
| 13 | U1511 dNTP | 200μl |
| 14 | U1515 dNTP | 1000μl |
| 15 | A1360 pGEM-T easy | 1.2ug |
| 16 | A3600 pGEM-T | 1.2ug |
| 17 | C1181 Random Primers | 20μg |
| 18 | N2511 Recombinant RNasin | 2500U |
| 19 | N2515 Recombinant RNasin | 10000U |
| 20 | N2611 RNasin Plus | 2500U |
| 21 | N2615 RNasin Plus | 10000U |
| 22 | N2111 RNasin | 2500U |
| 23 | N2115 RNasin | 10000U |
| 24 | M1871 TdT | 300U |
| 25 | M1875 TdT | 1500U |
| 26 | M2825 CIAP | 1000U |
| 27 | M1821 CIAP | 1000U |
| 28 | M2201 Klenow I | 150U |
| 29 | M2206 Klenow I | 500U |
| 30 | M4211 T4 DNA Polymerase | 100U |
| 31 | M4215 T4 DNA Polymerase | 500U |
| 32 | M4101 T4 Polynucleotide Kinase | 100U |
| 33 | M4103 T4 Polynucleotide Kinase | 1000U |
产品相关关键字： Promega试剂
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