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N2515 Recombinant RNasin Promega试剂?

原装正品，质量保证，现货供应

规格：10000u

N2515 Recombinant RNasin Promega试剂? 产品描述：

其它组分：

Recombinant RNasin® Ribonuclease Inhibitor【子货号：N251B，包装：1 x 10,000u，，运保温度：–20°C】

描述：

原厂资料：

RNases are ubiquitous, cause RNA degradation and can compromise RNA integrity. Recombinant RNasin® Inhibitor is a 50kDa protein that inhibits RNase A family and human placental RNases by noncovalently binding to RNases in a 1:1 ratio. Recombinant RNasin® Inhibitor does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, RNase H, RNase ONE™ Ribonuclease and enzymes for downstream applications such as GoScript™ Reverse Transcriptase, AMV/M-MLV reverse transcriptases, SP6, T7/T3 RNA polymerase, and Taq DNA polymerases. Learn more about our custom options for this product at: www.promega。。ｃｏｍ/custom/

Features - Benefits
 

Broad-Spectrum RNase Inhibition: Inhibits common eukaryotic RNases.
Compatible: Does not inhibit SP6, T7 or T3 RNA Polymerase; GoScript™ Reverse Transcriptase, AMV or M-MLV Reverse Transcriptase; or Taq DNA polymerase.
Flexible: Functional across wide pH range (pH 5–8) enabling use in many downstream assays.
Recombinant: Made in bacteria, minimizing the chances of human nucleic acid contamination.
Applications
RT-PCR and RT-qPCR.
 

Useful in any applications where eukaryotic RNase contamination is a potential problem.
Protection of mRNA in cDNA synthesis reactions.
In vitro transcription/translation.
Increase yield and activity of polysomes.
Improvement of in vitro virus replication.
Improvement of RNA translation in homologous systems.
Performing RNase-free antibody staining.
Enhancement of RNA yields from Riboprobe® System RNA synthesis reactions.
 

References
 

Blackburn, P. and Moore, S. (1982) In: The Enzymes, Vol. XV, Part B.
Blackburn, P. et al. (1977) J. Biol. Chem. 252, 5904–10.
Lee, F.S. et al. (1989) Biochemistry 28, 219–24.
Lee, F.S. et al. (1989) Biochemistry 28, 225–30.

注意事项：

QC Tests
SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease.

Source
Recombinant strain of E. coli (Cat.# N2511, N2515).

Storage Buffer
20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT and 50% (v/v) glycerol.

Storage Conditions
Store at –20°C.

Unit Definition
One unit is defined as the amount of RNasin® Ribonuclease Inhibitor required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2,3´-cyclic monophosphate by ribonuclease A.

For product intended use please see Patents & Disclaimers tab.

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产品相关关键字： Promega试剂