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新葡新京娱乐场>澳门新浦京8455com>>Promega试剂>N2515 Recombinant RNasin Promega试剂

N2515 Recombinant RNasin Promega试剂

N2515 Recombinant RNasin Promega试剂
N2515 Recombinant RNasin Promega试剂

N2515 Recombinant RNasin Promega试剂
规格:10000u
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产品先容

N2515 Recombinant RNasin Promega试剂?

原装正品,质量保证,现货供应

规格:10000u

N2515 Recombinant RNasin Promega试剂? 产品描述:

其它组分:

 

Recombinant RNasin® Ribonuclease Inhibitor【子货号:N251B,包装:1 x 10,000u,,运保温度:–20°C】

 

 

描述:

 

 

说明

 

天然的和重组的RNasin® 核酸酶抑制剂具有广谱的抑制RNase 活性的性质,包括抑制中性型(neutral type) 真核RNase。该抑制剂的分子量为50kDa,按1:1 的比例与RNase 非共价结合发挥抑制作用。RNasin® Ribonuclease Inhibitor 与RNase( 如RNase A) 结合的Ki 值大约是10-14M。Promega 提供两种不同的抑制剂:天然的RNasin® Ribonuclease Inhibitor 和重组的RNasin® Ribonuclease Inhibitor。两种产品均通过离子交换和亲和层析相结合的纯化方法纯化得到。

 

特点

 

•  抑制常见的真核RNase:RNasin® Ribonuclease Inhibitor 具有广谱RNase 抑制特性,包括 RNase A、 RNase B、 RNase C 和人类胎盘RNase,不抑制RNase T1、 S1 核酸酶、曲霉(Aspergillus ) 中的 RNase 以及RNase H、RNase ONE® Ribonuclease。

兼容性:RNasin® Ribonuclease Inhibitor 不抑制SP6、T7 或T3RNA Polymerase、ImProm-II™、AMV 或M-MLV Reverse Transcriptase 或Taq DNA Polymerase。

pH 范围宽:在较宽的pH 范围内(pH 5-8) 均可发挥作用。

 

 

 

原厂资料:

 

RNases are ubiquitous, cause RNA degradation and can compromise RNA integrity. Recombinant RNasin® Inhibitor is a 50kDa protein that inhibits RNase A family and human placental RNases by noncovalently binding to RNases in a 1:1 ratio. Recombinant RNasin® Inhibitor does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, RNase H, RNase ONE™ Ribonuclease and enzymes for downstream applications such as GoScript™ Reverse Transcriptase, AMV/M-MLV reverse transcriptases, SP6, T7/T3 RNA polymerase, and Taq DNA polymerases. Learn more about our custom options for this product at: www.promega。。com/custom/

 

Features - Benefits
 

Broad-Spectrum RNase Inhibition: Inhibits common eukaryotic RNases.
Compatible: Does not inhibit SP6, T7 or T3 RNA Polymerase; GoScript™ Reverse Transcriptase, AMV or M-MLV Reverse Transcriptase; or Taq DNA polymerase.
Flexible: Functional across wide pH range (pH 5–8) enabling use in many downstream assays.
Recombinant: Made in bacteria, minimizing the chances of human nucleic acid contamination.
Applications
RT-PCR and RT-qPCR.
 

Useful in any applications where eukaryotic RNase contamination is a potential problem.
Protection of mRNA in cDNA synthesis reactions.
In vitro transcription/translation.
Increase yield and activity of polysomes.
Improvement of in vitro virus replication.
Improvement of RNA translation in homologous systems.
Performing RNase-free antibody staining.
Enhancement of RNA yields from Riboprobe® System RNA synthesis reactions.
 

 

References
 

Blackburn, P. and Moore, S. (1982) In: The Enzymes, Vol. XV, Part B.
Blackburn, P. et al. (1977) J. Biol. Chem. 252, 5904–10.
Lee, F.S. et al. (1989) Biochemistry 28, 219–24.
Lee, F.S. et al. (1989) Biochemistry 28, 225–30.

 

 

注意事项:

 

 

QC Tests
SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease.

Source
Recombinant strain of E. coli (Cat.# N2511, N2515).

Storage Buffer
20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT and 50% (v/v) glycerol.

Storage Conditions
Store at –20°C.

Unit Definition
One unit is defined as the amount of RNasin® Ribonuclease Inhibitor required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2,3´-cyclic monophosphate by ribonuclease A.

For product intended use please see Patents & Disclaimers tab.

N2515 Recombinant RNasin Promega试剂? 相关产品列表:

Promega 系列
序号货号  名称 规格
1M1801  T4连接酶         100U 
2M1804      T4连接酶      500U
3M1701      M-MLV              10000U 
4M1705      M-MLV             50000U 
5M5101      AMV                    300U 
6M5108      AMV             1000U  
7M7741      Pfu                      100U
8M7745      Pfu            500U  
9C1101      Olig(dt)15 Primer          20ug 
10M3001      Go-Taq                   100U  
11M3005      Go-Taq                 500U    
12M3008      Go-Taq                2500U     
13U1511      dNTP                   200μl   
14U1515      dNTP                   1000μl  
15A1360      pGEM-T easy              1.2ug   
16A3600      pGEM-T                     1.2ug 
17C1181      Random Primers              20μg  
18N2511      Recombinant RNasin           2500U 
19N2515      Recombinant RNasin      10000U  
20N2611      RNasin Plus                2500U  
21N2615      RNasin Plus              10000U   
22N2111      RNasin                   2500U     
23N2115      RNasin                  10000U   
24M1871      TdT                       300U 
25M1875      TdT                      1500U 
26M2825      CIAP                       1000U  
27M1821      CIAP           1000U            
28M2201      Klenow I      150U        
29M2206      Klenow I        500U           
30M4211      T4 DNA Polymerase  100U      
31M4215      T4 DNA Polymerase   500U          
32M4101      T4 Polynucleotide Kinase 100U          
33M4103      T4 Polynucleotide Kinase  1000U         

产品相关关键字: Promega试剂

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 相关同类产品:
Promega普洛麦格C1101 Oligo(dT)15 Primer 20ug 现货  GoTaq DNA Polymerase 100u 5u/μl Promega普洛麦格M3001现货  GoTaq DNA Polymerase 500u 5u/μl Promega普洛麦格M3005现货 
M4103 T4 Polynucleotide Kinase Promega试剂  M4101 T4 Polynucleotide Kinase Promega试剂  M4215 T4 DNA Polymerase Promega试剂 
M4211 T4 DNA Polymerase Promega试剂  M2206 Klenow I Promega试剂  M2201 Klenow I Promega试剂 

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